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KMID : 0617719980080010041
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Journal of Wonkwang Dental Research Institute
1998 Volume.8 No. 1 p.41 ~ p.53
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Effects of Seed of Cart ham us tinctorius L. on Gingival fibroblasts, Periodontal ligament cells and Osteoblasts
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Abstract
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The purpose of this study was to evaluate the effects of Carthamus tinctorius L on gingival fibroblasts, periodontal ligament cells and osteoblasts. Periodontal ligament cells and gingival fibroblasts were primarily from extracted premolar with non-periodontal diseases. 0steoblasts were getten from calvariae of rat. Cells were cultured with DMEM at 371c, 5% C02, 100% humidity incubator. MTT assay and collagen synthesis were used as a method of this paper.
The obtained results were as follows
1. The cellular activity of gingival fibroblasts was significantly increased in 10-4g/ml of Carthamus tinctorius L. at the culture of the first, the second and the third day.
2. The cellular activity of periodontal ligament cells was significantly increased in 10-3g/ml of Carthamus tinctorius L. at the culture of the first, the second and the third day.
3. The cellular activity of osteoblasts was increased and maintained in 10-3g/ml and 10-4g/ml of Carthamus tinctorius L. but was significantly decreased over the level of 10-3g/ml.
4. The collagen synthesis of periodontal ligament cells was significantly more increased in 101´g/ml than that of the control. and the collagen synthesis of osteoblasts was significantly increased in
10-3 g/ml too.
These findings suggest that increased cellular activity and collagen synthesis by Carthamus tinctorius L may help the periodontal regeneration and reconstruction.
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